유전자 질환 발현 수수께끼 풀렸다 AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation

출처 : Nucleic Acids Research
등록일 : 2021-09-01
조회 : 124

<div style="text-align: justify;" align="justify"><b><span style="font-family: 돋움, dotum; font-size: 11pt; color: rgb(58, 50, 195);"><span style="color: rgb(0, 0, 0); font-size: 11pt; font-family: 돋움, dotum;"></span></span></b><a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab728/6362091#300082212" target="_blank"><span style="font-size: 11pt; font-family: 돋움, dotum;">👉 논문명:&nbsp; AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation(Nucleic Acids Research</span></a><a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab728/6362091#300082212" target="_blank"><span style="font-size: 11pt; font-family: 돋움, dotum;">, 2021.09.01)</span></a></div><div style="text-align: justify;" align="justify"><b><span style="font-size: 11pt; font-family: 돋움, dotum;"><br></span></b></div><div style="text-align: justify;" align="justify"><b><span style="font-size: 11pt; font-family: 돋움, dotum;">Abstract</span></b></div><div style="text-align: justify;" align="justify"><b><span style="font-size: 10pt; font-family: 돋움, dotum;"><br></span></b></div><div style="text-align: justify;" align="justify"><span style="font-size: 11pt; font-family: 돋움, dotum;">I-motif or C4 is a four-stranded DNA structure with a protonated cytosine:cytosine base pair (C+:C) found in cytosine-rich sequences. We have found that oligodeoxynucleotides containing adenine and cytosine repeats form a stable secondary structure at a physiological pH with magnesium ion, which is similar to i-motif structure, and have named this structure ‘adenine:cytosine-motif (AC-motif)’. AC-motif contains C+:C base pairs intercalated with putative A+:C base pairs between protonated adenine and cytosine. By investigation of the AC-motif present in the CDKL3 promoter (AC-motifCDKL3), one of AC-motifs found in the genome, we confirmed that AC-motifCDKL3 has a key role in regulating CDKL3 gene expression in response to magnesium. This is further supported by confirming that genome-edited mutant cell lines, lacking the AC-motif formation, lost this regulation effect. Our results verify that adenine-cytosine repeats commonly present in the genome can form a stable non-canonical secondary structure with a non-Watson–Crick base pair and have regulatory roles in cells, which expand non-canonical DNA repertoires.</span></div><div style="text-align: justify;" align="justify"><a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab728/6362091#300082212" target="_blank"><br></a></div><div style="text-align: justify;" align="justify"><div style="text-align: justify;" align="justify"><font face="돋움, dotum"><span style="font-size: 14.6667px;"><br></span></font></div><div style="text-align: justify;" align="justify"><font face="돋움, dotum"><span style="font-size: 14.6667px;">👉</span></font><span style="font-size: 11pt; font-family: 돋움, dotum;">논문보러가기:</span><a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab728/6362091#300082212" target="_blank"><span style="font-size: 11pt; font-family: 돋움, dotum;">https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab728/6362091#300082212</span></a></div></div><div style="text-align: justify;" align="justify"><br></div><div style="text-align: justify;" align="justify"><span style="font-family: 돋움, dotum; font-size: 11pt; color: rgb(58, 50, 225);">※ 자세한 내용은 URL을 확인하시기 바랍니다.</span></div>

URL : https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab728/6362091#300082212

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